Global Lifetime Analysis

Written by Chavdar Slavov

Typically, in time-resolved spectroscopy the multiple kinetic traces recorded in dependence of wavelength or other experimental variable are analyzed simultaneouslyi in a procedure called global analysis. The most common global analysis procedure is global lifetime analysis (GLA), where the experimental data is approximated by a discrete sum-of-exponentials function. The global parameters spanning the dataset are the lifetimes, while the pre-exponential amplitudes are determined for each kinetic trace. GLA results in the so-called decays-associated spectra (DAS), where the pre-exponential amplitudes for each lifetime component are plotted against an experimental variable, e.g. detection wavelength. The DAS is a compact representation of the kinetic information in the data.

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Details in:

Implementation and evaluation of data analysis strategies for time-resolved optical spectroscopy

Chavdar Slavov, Helvi Hartmann, Josef Wachtveitl, Analytical Chemistry, 2015, 87, 2328

See also:

The global analysis of fluorescence intensity and anisotropy decay data: second generation thoery and programs

Beechem, J. M., Gratton, E., Ameloot, M., Knutson, J. R., Brand, Topics in Fluorescence Spectroscopy, Lakowicz, J. R., Ed. 1991, 241

Data Analysis of Time-Resolved Measurements

Holzwarth, A. R., Biophysical Techniques in Photosynthesis. Advances in Photosynthesis Research, Amesz, J., Hoff, A. J., Eds., 1996, 75

Global and target analysis of time-resolved spectra

van Stokkum, I.H.M., Larsen, D.S., van Grondelle, R., BBA - Bioenergetics, 2004, 1657, 82

Modelling Multi-Pulse Population Dynamics from Ultrafast Spectroscopy

van Wilderen, L.J.G.W., Lincoln, C.N., van Thor, J.J., PLoS ONE, 2011, 6, e17373