Global Target Analysis

Written by Chavdar Slavov

Global target analysis (GTA) is the more general case of global analysis, where kinetic models are directly tested for compliance with the time-resolved data. GTA is essential, because kinetic modeling is the only quantitative way to reveal reaction mechanisms. In essence, GTA consists of devising a kinetic reaction scheme based on previous knowledge about the studied system and certain assumptions. The kinetic scheme is then represented by a system of differential equations. The solution of such a system of differential equations for first-order kinetics is a sum-of-exponentials function similar to the one used in GLA, with the difference that now the lifetimes depend in a complex manner, defined by the kinetic model, on the kinetic rates. An important parameter that emerges from GTA is the species-associated spectrum (SAS), which physically represents the stationary spectra of the model compartments as if they were measured separately.

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Details in:

Implementation and evaluation of data analysis strategies for time-resolved optical spectroscopy

Chavdar Slavov, Helvi Hartmann, Josef Wachtveitl, Analytical Chemistry, 2015, 87, 2328

See also:

The global analysis of fluorescence intensity and anisotropy decay data: second generation thoery and programs

Beechem, J. M., Gratton, E., Ameloot, M., Knutson, J. R., Brand, Topics in Fluorescence Spectroscopy, Lakowicz, J. R., Ed. 1991, 241

Data Analysis of Time-Resolved Measurements

Holzwarth, A. R., Biophysical Techniques in Photosynthesis. Advances in Photosynthesis Research, Amesz, J., Hoff, A. J., Eds., 1996, 75

Global and target analysis of time-resolved spectra

van Stokkum, I.H.M., Larsen, D.S., van Grondelle, R., BBA - Bioenergetics, 2004, 1657, 82

Modelling Multi-Pulse Population Dynamics from Ultrafast Spectroscopy

van Wilderen, L.J.G.W., Lincoln, C.N., van Thor, J.J., PLoS ONE, 2011, 6, e17373