Lifetime Density Analysis
- Written by Chavdar Slavov
The great number of spatial, energetic and temporal degrees of freedom of the studied molecule ensembles and their matrix produces a continuous distribution of individual exponential decays. In this respect, the approximation of the data profile by a discrete set of exponentials (GLA and GTA) must be interpreted as a curve parameterization, concealing potential contribution of a larger number of underlying decays. Within lifetime density analysis (LDA) a quasi-continuous sum-of-exponents function is used to perform model-independent analysis of the experimental data. The methods yields a lifetime density/distribution map (LDM) which gives a comprehensive overview of the kinetics.
Implementation and evaluation of data analysis strategies for time-resolved optical spectroscopyChavdar Slavov, Helvi Hartmann, Josef Wachtveitl, Analytical Chemistry, 2015, 87, 2328
Recovery of underlying distributions of lifetimes from fluorescence decay dataJames, D. R., Ware, W. R.,Chemical Physics Letters, 1986, 126, 7
Analyzing the Distribution of Decay Constants in Pulse-Fluorimetry Using the Maximum Entropy MethodLivesey, A. K., Brochon, J. C., Biophysical Journal, 1987, 52, 693
Distribution of event times in time-resolved fluorescence: The exponential series approach--algorithm, regularization, analysisLandl, G., Langthaler, T., Englt, H. W., Kauffmann, H. F., Journal of Computational Physics, 1991, 95, 1
Carotenoid-to-Chlorophyll Energy Transfer in Recombinant Major Light-Harvesting Complex (LHCII) of Higher Plants. I. Femtosecond Transient Absorption MeasurementsCroce, R., Müller, M. G., Bassi, R., Holzwarth, A. R., Biophysical Journal, 2001, 80, 901