Lifetime Density Analysis

Written by Chavdar Slavov

The great number of spatial, energetic and temporal degrees of freedom of the studied molecule ensembles  and their matrix produces a continuous distribution of individual exponential decays. In this respect, the approximation of the data profile by a discrete set of exponentials (GLA and GTA) must be interpreted as a curve parameterization, concealing potential contribution of a larger number of underlying decays. Within lifetime density analysis (LDA) a quasi-continuous sum-of-exponents function is used to perform model-independent analysis of the experimental data. The methods yields a lifetime density/distribution map (LDM) which gives a comprehensive overview of the kinetics.

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Details in:

Implementation and evaluation of data analysis strategies for time-resolved optical spectroscopy

Chavdar Slavov, Helvi Hartmann, Josef Wachtveitl, Analytical Chemistry, 2015, 87, 2328

See also:

Recovery of underlying distributions of lifetimes from fluorescence decay data

James, D. R., Ware, W. R.,Chemical Physics Letters, 1986, 126, 7

Analyzing the Distribution of Decay Constants in Pulse-Fluorimetry Using the Maximum Entropy Method

Livesey, A. K., Brochon, J. C., Biophysical Journal, 1987, 52, 693

Distribution of event times in time-resolved fluorescence: The exponential series approach--algorithm, regularization, analysis

Landl, G., Langthaler, T., Englt, H. W., Kauffmann, H. F., Journal of Computational Physics, 1991, 95, 1

Carotenoid-to-Chlorophyll Energy Transfer in Recombinant Major Light-Harvesting Complex (LHCII) of Higher Plants. I. Femtosecond Transient Absorption Measurements

Croce, R., Müller, M. G., Bassi, R., Holzwarth, A. R., Biophysical Journal, 2001, 80, 901